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A monohybrid cross is a cross between two organisms with different variations at one genetic locus of interest. The character(s) being studied in a monohybrid cross are governed by two or multiple variations for a single location of a gene. Then carry out such a cross, each parent is chosen to be homozygous or true breeding for a given trait (locus). When a cross satisfies the conditions for a monohybrid cross, it is usually detected by a characteristic distribution of second-generation (F2) offspring that is sometimes called the monohybrid ratio.